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ProductsBack/RFC1 / RFC Antibody

RFC1 / RFC Antibody

LS-C331592
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetRFC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RFC1 / RFC Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 128kDa, while the observed MW by Western blot was 130kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 128kDa, while the observed MW by Western blot was 130kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5981
  • Target name
    RFC1
  • Target description
    replication factor C subunit 1
  • Target synonyms
    A1, CANVAS, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1, A1 140 kDa subunit, DNA-binding protein PO-GA, MHC binding factor, beta, RF-C 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa subunit, replication factor C large subunit, replication factor C1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFC1 using anti-RFC1 antibody (PA1716). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody (Catalog # PA1716) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150 kDa. The expected band size for RFC1 is at 128 kDa.
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