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ProductsBack/RHOT1 antibody, N-term
IHC-P analysis of human muscle tissue using GTX46625 RHOT1 antibody at 4.0-8.0microg/ml.
IHC-P analysis of human muscle tissue using GTX46625 RHOT1 antibody at 4.0-8.0microg/ml.
IHC-P analysis of human muscle tissue using GTX46625 RHOT1 antibody at 4.0-8.0microg/ml.

RHOT1 antibody, N-term

GTX46625
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRHOT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RHOT1 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55288
  • Target name
    RHOT1
  • Target description
    ras homolog family member T1
  • Target synonyms
    ARHT1, MIRO-1, MIRO1, mitochondrial Rho GTPase 1, mitochondrial Rho (MIRO) GTPase 1, rac-GTP binding protein-like protein, ras homolog gene family, member T1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXI2
  • Protein Name
    Mitochondrial Rho GTPase 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO1/RHOT1 antigen affinity purified polyclonal antibody (Catalog # A05928-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO1/RHOT1 at approximately 71 kDa. The expected band size for MIRO1/RHOT1 is at 71 kDa.
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