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ProductsBack/RICTOR (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1G11]
Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3

RICTOR (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1G11]

AM20222PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRICTOR
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    RICTOR (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1G11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G11
  • Gene ID253260
  • Target name
    RICTOR
  • Target description
    RPTOR independent companion of MTOR complex 2
  • Target synonyms
    AVO3, PIA, hAVO3, rapamycin-insensitive companion of mTOR, AVO3 homolog, TORC2-specific protein AVO3, pianissimo
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ6R327
  • Protein Name
    Rapamycin-insensitive companion of mTOR
  • Scientific Description
    RICTOR (incl. pos. control) mouse monoclonal antibody, clone 1G11, Purified
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RICTOR using anti-RICTOR antibody (A03195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RICTOR antigen affinity purified polyclonal antibody (Catalog # A03195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RICTOR at approximately 230KD. The expected band size for RICTOR is at 192KD.
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