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ProductsBack/RIP1 / RALBP1 Antibody (aa403-499)

RIP1 / RALBP1 Antibody (aa403-499)

LS-C296182
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRALBP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RIP1 / RALBP1 Antibody (aa403-499)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10928
  • Target name
    RALBP1
  • Target description
    ralA binding protein 1
  • Target synonyms
    RIP1, RLIP1, RLIP76, ralA-binding protein 1, 76 kDa Ral-interacting protein, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase, ral-interacting protein 1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.
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