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ProductsBack/RNF43 Antibody - C-terminal region

RNF43 Antibody - C-terminal region

OAAB09509
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNF43
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    RNF43 Antibody - C-terminal region
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:2000 WB: 1:1000 IHC-P: 1:10~50
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID54894
  • Target name
    RNF43
  • Target description
    ring finger protein 43
  • Target synonyms
    RNF124, SSPCS, URCC, E3 ubiquitin-protein ligase RNF43, RING-type E3 ubiquitin transferase RNF43
  • Host
    Rabbit
  • Protein IDQ68DV7
  • Protein Name
    E3 ubiquitin-protein ligase RNF43
  • Scientific Description
    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (Catalog # A01694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86KD. The expected band size for RNF43 is at 86KD.
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