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ProductsBack/RNF86 (TRIM2) Mouse Monoclonal Antibody [Clone ID: OTI2B2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY414662 (100ug) and LC414662 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY414662 (100ug) and LC414662 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY414662 (100ug) and LC414662 (20ug) can be purchased separately from OriGene.

RNF86 (TRIM2) Mouse Monoclonal Antibody [Clone ID: OTI2B2]

CF501552
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRIM2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    RNF86 (TRIM2) Mouse Monoclonal Antibody [Clone ID: OTI2B2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2B2
  • Gene ID23321
  • Target name
    TRIM2
  • Target description
    tripartite motif containing 2
  • Target synonyms
    CMT2R, RNF86, tripartite motif-containing protein 2, Charcot-Marie-Tooth disease, type 2R, E3 ubiquitin-protein ligase TRIM2, RING finger protein 86, RING-type E3 ubiquitin transferase TRIM2, tripartite motif protein TRIM2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9C040
  • Protein Name
    Tripartite motif-containing protein 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TRIM2 mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TRIM2 using anti-TRIM2 antibody (A08957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM2 antigen affinity purified polyclonal antibody (Catalog # A08957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM2 at approximately 82 kDa. The expected band size for TRIM2 is at 82 kDa.
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