![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_20200117_WB_competitor_watermark_w_23061202_820.webp)
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
ROCK1 + ROCK2 antibody [GT464]
GTX629972
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetROCK1
Overview
- SupplierGeneTex
- Product NameROCK1 + ROCK2 antibody [GT464]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT464
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6093
- Target nameROCK1
- Target descriptionRho associated coiled-coil containing protein kinase 1
- Target synonymsP160ROCK, ROCK-I, rho-associated protein kinase 1, p160 ROCK-1, renal carcinoma antigen NY-REN-35
- HostMouse
- IsotypeIgG2b
- Protein IDO75116
- Protein NameRho-associated protein kinase 2
- Scientific DescriptionThis gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg BCL-1 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000 ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg BCL-1 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract F. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_WB_M_w_23061202_493.webp)
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:3000. Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:3000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_20160728_WB_shRNA_watermark_w_23061202_210.webp)
![ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000 ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_WB_R_w_23061202_266.webp)
![Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:500. Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [GT464] (GTX629972) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_20160616_WB_Cisplatin_w_23061202_349.webp)
![ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000 ROCK1 + ROCK2 antibody [GT464] detects ROCK1 + ROCK2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT464] (GTX629972) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX629972/GTX629972_41484_WB_w_23061202_982.webp)



![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [N1N2], N-term (GTX113266) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113266/GTX113266_44608_20220311_WB_w_23060501_218.webp)
![WB analysis of HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate using GTX60535 ROCK1 antibody [1H4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60535/GTX60535_20170912_WB_w_23061123_987.webp)
![ROCK1 + ROCK2 antibody [GT261] detects ROCK1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 + ROCK2 antibody [GT261] (GTX629971) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX629971/GTX629971_41484_WB_w_23061202_231.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (GTX637125) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637125/GTX637125_T-44739_20230707_WB_multiple_B_23071223_441.webp)


