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ProductsBack/ROCK1 Polyclonal Antibody Cy5 Conjugated

ROCK1 Polyclonal Antibody Cy5 Conjugated

MBS9460412
MyBioSource
Product group Antibodies
Price on request
0.1 ml
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Overview

  • Supplier
    MyBioSource
  • Product Name
    ROCK1 Polyclonal Antibody Cy5 Conjugated
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Other Conjugate
  • Estimated Purity
    Purified by Protein A
  • Isotype
    IgG
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (Catalog # A00722-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 160 kDa. The expected band size for ROCK1 is at 158 kDa.
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