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ProductsBack/ROCK2 (phospho Ser1366) antibody

ROCK2 (phospho Ser1366) antibody

GTX122651
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
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Product group Antibodies
TargetROCK2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ROCK2 (phospho Ser1366) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9475
  • Target name
    ROCK2
  • Target description
    Rho associated coiled-coil containing protein kinase 2
  • Target synonyms
    p164 ROCK-2; rho-associated protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; ROCK-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75116
  • Protein Name
    Rho-associated protein kinase 2
  • Scientific Description
    The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cocaine and habit training cause dendritic spine rearrangement in the prelimbic cortex.
    Read more
  • Cannabinoid CB(1) receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice.
    Read more
  • TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent manner. Zheng H et al., 2021 Dec, Acta Pharm Sin B
    Read more
  • RAB7A Regulates Vimentin Phosphorylation through AKT and PAK. Romano R et al., 2021 May 6, Cancers (Basel)
    Read more
  • ROCK2 inhibition enhances the thermogenic program in white and brown fat tissue in mice. Wei L et al., 2020 Jan, FASEB J
    Read more
  • Phosphorylation of ARHGAP19 by CDK1 and ROCK regulates its subcellular localization and function during mitosis. Marceaux C et al., 2018 Mar 2, J Cell Sci
    Read more
  • ARHGEF10 knockout inhibits platelet aggregation and protects mice from thrombus formation. Lu DH et al., 2017 Oct, J Thromb Haemost
    Read more
  • Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus. Wang W et al., 2018 Jul 1, Cereb Cortex
    Read more
  • Induction of endothelium-dependent constriction of mesenteric arteries in endotoxemic hypotensive shock. Tseng TL et al., 2016 Apr, Br J Pharmacol
    Read more
  • 14-3-3tau promotes breast cancer invasion and metastasis by inhibiting RhoGDIalpha. Xiao Y et al., 2014 Jul, Mol Cell Biol
    Read more

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Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (A01023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7 : rat brain tissue lysates, Lane 8 : rat PC-12 whole cell lysates, Lane 9 : mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # A01023-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161KD. The expected band size for ROCK2 is at 161KD.
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