More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ROCK2 (phospho Ser1366) antibody
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ROCK2 (phospho Ser1366) antibody

GTX122651
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetROCK2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ROCK2 (phospho Ser1366) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.06 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9475
  • Target name
    ROCK2
  • Target description
    Rho associated coiled-coil containing protein kinase 2
  • Target synonyms
    ROCK-II, rho-associated protein kinase 2, p164 ROCK-2, rho-associated, coiled-coil-containing protein kinase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75116
  • Protein Name
    Rho-associated protein kinase 2
  • Scientific Description
    The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
ROCK2 Monoclonal AntibodyCAB2395
ReactivityHuman
TargetROCK2
  • SizePrice
Western Blot analysis of RAT-MUSCLE cells using Rock-2 Polyclonal Antibody
Product group Antibodies
Cusabio
ROCK2 AntibodyCSB-PA004018
ApplicationsWestern Blot, ELISA
ReactivityHuman, Monkey, Mouse, Rat
TargetROCK2
  • SizePrice
Anti-ROCK2 Antibody
Product group Antibodies
Antibodies.com
Anti-ROCK2 AntibodyA97284
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (A01023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7 : rat brain tissue lysates, Lane 8 : rat PC-12 whole cell lysates, Lane 9 : mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # A01023-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161KD. The expected band size for ROCK2 is at 161KD.
Product group Antibodies
Boster Bio
Anti-ROCK2 Antibody Picoband(r)A01023-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetROCK2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ROCK2 AntibodyLS-C831703
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetROCK2
  • SizePrice
Immunohistochemical staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.
Product group Antibodies
Atlas Antibodies
Anti-ROCK2 AntibodyHPA007459
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetROCK2
  • SizePrice
Product group Antibodies
Biomatik
ROCK2 Polyclonal AntibodyCAC14876
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetROCK2
  • SizePrice
ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ROCK2 protein stained by ROCK2 antibody (GTX102619) diluted at 1:500. Blue: Hoechst 33342 staining.
Product group Antibodies
GeneTex
ROCK2 antibodyGTX102619
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetROCK2
  • SizePrice
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal (GTX108247) diluted at 1:500 and competitor's antibody (sc-1851) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
Product group Antibodies
GeneTex
ROCK2 antibody [N3C1], InternalGTX108247
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetROCK2
  • SizePrice
ROCK2 antibody detects ROCK2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ROCK2 protein stained by ROCK2 antibody (GTX122652) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Product group Antibodies
GeneTex
ROCK2 antibodyGTX122652
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetROCK2
  • SizePrice