FACS analysis of HeLa cells using GTX83695 RPA32 antibody [7C12]. Red : Primary antibody Blue : Negative control antibody
![FACS analysis of HEK293T cells transfected with either RPA32 plasmid(Red) or empty vector control plasmid(Blue) using GTX83695 RPA32 antibody [7C12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_124_FACS_w_23061420_643.webp "FACS analysis of HEK293T cells transfected with either RPA32 plasmid(Red) or empty vector control plasmid(Blue) using GTX83695 RPA32 antibody [7C12].")
![IHC-P analysis of human colon adenocarcinoma tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1722_IHC-P_w_23061420_300.webp "IHC-P analysis of human colon adenocarcinoma tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1723_IHC-P_w_23061420_946.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human breast tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1724_IHC-P_w_23061420_869.webp "IHC-P analysis of human breast tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human colon tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1725_IHC-P_w_23061420_513.webp "IHC-P analysis of human colon tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human endometrium tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1726_IHC-P_w_23061420_908.webp "IHC-P analysis of human endometrium tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1727_IHC-P_w_23061420_709.webp "IHC-P analysis of human kidney tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1728_IHC-P_w_23061420_807.webp "IHC-P analysis of human ovary tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83695/GTX83695_1729_IHC-P_w_23061420_992.webp "IHC-P analysis of human pancreas tissue using GTX83695 RPA32 antibody [7C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
FACS analysis of HeLa cells using GTX83695 RPA32 antibody [7C12]. Red : Primary antibody Blue : Negative control antibody
RPA32 antibody [7C12]
GTX83695
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetRPA2
Overview
- SupplierGeneTex
- Product NameRPA32 antibody [7C12] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:4000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7C12
- Concentration0.75 mg/ml
- ConjugateUnconjugated
- Gene ID6118
- Target nameRPA2
- Target descriptionreplication protein A2
- Target synonymsREPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
- HostMouse
- IsotypeIgG2a
- Protein IDP15927
- Protein NameReplication protein A 32 kDa subunit
- Scientific DescriptionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair - it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
- ReactivityCanine, Human, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203