RPA32 antibody [RPA34-19]

GeneTex

RPA32 antibody [RPA34-19]

9

For IF: Use at a concentration of 1 - 5 microg/ml. For IHC-P: Use at a concentration of 2.5 - 5 microg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 5 microg/ml. Predicted molecular weight: RPA, is a multisubunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein. Optimal dilutions/concentrations should be determined by the researcher.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

RPA34-19

0.1 mg/ml

Unconjugated

RPA2

replication protein A2

REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32

Mouse

IgG2a

Replication protein A 32 kDa subunit

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

2°C to 8°C

12352203