Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20221216_WB_Z_22122018_765.webp "Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20221228_IHC-P_M_22122901_353.webp "RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20221228_IHC-P_R_22122901_560.webp "RPB2 antibody [HL1042] detects RPB2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20230324_IHC-Wm_Z_1_23041023_246.webp "RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC")
![RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20230324_IHC-Wm_Z_23041023_363.webp "RPB2 antibody [HL1042] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC")
![293T whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20210611_WB_w_23061202_496.webp "293T whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![RPB2 antibody [HL1042] detects RPB2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20220506_IHC-P_w_23061202_483.webp "RPB2 antibody [HL1042] detects RPB2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RPB2 stained by RPB2 antibody [HL1042] (GTX635976) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20220408_WB_R_w_23061202_241.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20220401_WB_M_w_23061202_651.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RPB2 antibody [HL1042]
GTX635976
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
TargetPOLR2B
Overview
- SupplierGeneTex
- Product NameRPB2 antibody [HL1042]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1042
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5431
- Target namePOLR2B
- Target descriptionRNA polymerase II subunit B
- Target synonymsPOL2RB, RPB2, hRPB140, DNA-directed RNA polymerase II subunit RPB2, 3'-5' exoribonuclease, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II second largest subunit, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA-directed RNA polymerase II subunit RPB2, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, polymerase (RNA) II subunit B
- HostRabbit
- IsotypeIgG
- Protein IDP30876
- Protein NameDNA-directed RNA polymerase II subunit RPB2
- Scientific DescriptionThis gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
- ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221209_WB_D_C_22121123_648.webp)
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