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RPS19 Antibody (YA1752, Rabbit)

HY-P82007
MedChem Express
TargetRPS19
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RPS19 Antibody (YA1752, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6223
  • Target name
    RPS19
  • Target description
    ribosomal protein S19
  • Target synonyms
    DBA, DBA1, LOH19CR1, S19, eS19, small ribosomal subunit protein eS19, 40S ribosomal protein S19, loss of heterozygosity on chromosome 19, region 1, loss of heterozygosity, 19, chromosomal region 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RPS19 Antibody (YA1752) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1752), targeting RPS19, with a predicted molecular weight of 16 kDa (observed band size: 16 kDa). RPS19 Antibody (YA1752) can be used for WB, IHC-P, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS19 using anti-RPS19 antibody (A02343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS19 antigen affinity purified polyclonal antibody (Catalog # A02343-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS19 at approximately 16 kDa. The expected band size for RPS19 is at 16 kDa.
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