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Western Blot analysis of HepG2 cells using Ribosomal Protein S3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Ribosomal Protein S3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Ribosomal Protein S3 Polyclonal Antibody

RPS3 Antibody

CSB-PA004002
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPS3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6188
  • Target name
    RPS3
  • Target description
    ribosomal protein S3
  • Target synonyms
    S3, uS3, small ribosomal subunit protein uS3, 40S ribosomal protein S3, IMR-90 ribosomal protein S3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23396
  • Protein Name
    40S ribosomal protein S3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human U2OS whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human PC-3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.
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