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RPS3 / Ribosomal Protein S3 Antibody

LS-C770370
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRPS3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPS3 / Ribosomal Protein S3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:5000), IHC (1:25 - 1:100), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6188
  • Target name
    RPS3
  • Target description
    ribosomal protein S3
  • Target synonyms
    40S ribosomal protein S3; IMR-90 ribosomal protein S3; S3; small ribosomal subunit protein uS3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human U2OS whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human PC-3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.
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