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ProductsBack/RPS3 Rabbit Polyclonal Antibody
WB Suggested Anti-RPS3 Antibody Titration: 1.0 ug/ml Positive Control: A549 Whole CellRPS3 is supported by BioGPS gene expression data to be expressed in A549
WB Suggested Anti-RPS3 Antibody Titration: 1.0 ug/ml Positive Control: A549 Whole CellRPS3 is supported by BioGPS gene expression data to be expressed in A549
WB Suggested Anti-RPS3 Antibody Titration: 1.0 ug/ml Positive Control: A549 Whole CellRPS3 is supported by BioGPS gene expression data to be expressed in A549

RPS3 Rabbit Polyclonal Antibody

TA358515
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetRPS3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RPS3 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6188
  • Target name
    RPS3
  • Target description
    ribosomal protein S3
  • Target synonyms
    40S ribosomal protein S3; IMR-90 ribosomal protein S3; S3; small ribosomal subunit protein uS3
  • Host
    Rabbit
  • Protein IDP23396
  • Protein Name
    40S ribosomal protein S3
  • Scientific Description
    RPS3 Antibody - middle region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: human K562 whole cell lysate, Lane 5: human U2OS whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human PC-3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.
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