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ProductsBack/RPS6 (phospho Ser235/236) antibody
WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of HeLa cells treated with anisomycin (Lane 2-5) using GTX12864 RPS6 (phospho Ser235/236) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

RPS6 (phospho Ser235/236) antibody

GTX12864
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetRPS6
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPS6 (phospho Ser235/236) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6194
  • Target name
    RPS6
  • Target description
    ribosomal protein S6
  • Target synonyms
    S6, eS6, small ribosomal subunit protein eS6, 40S ribosomal protein S6, phosphoprotein NP33
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62753
  • Protein Name
    Small ribosomal subunit protein eS6
  • Scientific Description
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Goodson WH 3rd, Luciani MG, Sayeed SA, et al. Activation of the mTOR pathway by low levels of xenoestrogens in breast epithelial cells from high-risk women. Carcinogenesis. 2011,32(11):1724-33. doi: 10.1093/carcin/bgr196
    Read this paper

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