Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640988/GTX640988_T-45537_20240927_WB_treatment_TPA_24100318_425.webp "Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![RSK3 (phospho Thr356/Ser360) antibody [HL3299] detects RSK3 (phospho Thr356/Ser360) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RSK3 (phospho Thr356/Ser360) stained by RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640988/GTX640988_T-45537_20241004_ICC_IF_treatment_TPA_24110700_185.webp "RSK3 (phospho Thr356/Ser360) antibody [HL3299] detects RSK3 (phospho Thr356/Ser360) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RSK3 (phospho Thr356/Ser360) stained by RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 (phospho Thr356/Ser360) antibody [HL3299] (GTX640988) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RSK3 (phospho Thr356/Ser360) antibody [HL3299]
GTX640988
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRPS6KA2
Overview
- SupplierGeneTex
- Product NameRSK3 (phospho Thr356/Ser360) antibody [HL3299]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3299
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6196
- Target nameRPS6KA2
- Target descriptionribosomal protein S6 kinase A2
- Target synonymsHU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3, ribosomal protein S6 kinase alpha-2, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, mitogen-activated protein kinase-activated protein kinase 1C, ribosomal S6 kinase 3, ribosomal protein S6 kinase, 90kDa, polypeptide 2
- HostRabbit
- IsotypeIgG
- Protein IDQ15349
- Protein NameRibosomal protein S6 kinase alpha-2
- Scientific DescriptionThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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