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Western blot analysis of extracts of various cell lines using RUFY3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RUFY3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RUFY3 Polyclonal Antibody at dilution of 1:3000.

RUFY3 Polyclonal Antibody

E-AB-64364
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RUFY3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of RUFY3 using anti-RUFY3 antibody (A09453-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY3 antigen affinity purified polyclonal antibody (Catalog # A09453-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY3 at approximately 53-65 kDa. The expected band size for RUFY3 is at 53-65 kDa.
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