Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638215/GTX638215_T-44942_20230210_WB_M_23021401_270.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638215/GTX638215_T-44942_20230210_WB_R_23021401_951.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638215/GTX638215_T-44942_20230303_IHC-P_multiple_M_23031402_536.webp "RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638215/GTX638215_T-44942_20230428_ICC_IF_23050918_613.webp "RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![RUNX1 antibody [HL2211] detects RUNX1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638215/GTX638215_45012_20231117_ICC_IF_23121122_394.webp "RUNX1 antibody [HL2211] detects RUNX1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (GTX638215) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (GTX638215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RUNX1 antibody [HL2211]
GTX638215
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRUNX1
Overview
- SupplierGeneTex
- Product NameRUNX1 antibody [HL2211]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2211
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID861
- Target nameRUNX1
- Target descriptionRUNX family transcription factor 1
- Target synonymsAML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha, runt-related transcription factor 1, AML1-ETO fusion, AML1-ETO fusion protein, AML1-EVI-1 fusion protein, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, acute myeloid leukemia 1 protein, core-binding factor, runt domain, alpha subunit 2, mutant RUNX1, oncogene AML-1, polyomavirus enhancer-binding protein 2 alpha B subunit, runt related transcription factor 1
- HostRabbit
- IsotypeIgG
- Protein IDQ01196
- Protein NameRunt-related transcription factor 1
- Scientific DescriptionCore binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
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