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ProductsBack/RUNX1T1 / ETO Antibody

RUNX1T1 / ETO Antibody

LS-C497925
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRUNX1T1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RUNX1T1 / ETO Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 44kDa/48kDa/63kDa/64kDa/67kDa/68kDa, while the observed MW by Western blot was 65kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:200 - 1:1000) The predicted MW is 44kDa/48kDa/63kDa/64kDa/67kDa/68kDa, while the observed MW by Western blot was 65kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID862
  • Target name
    RUNX1T1
  • Target description
    RUNX1 partner transcriptional co-repressor 1
  • Target synonyms
    AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2, protein CBFA2T1, RUNX1 translocation partner 1, acute myelogenous leukemia 1 translocation 1, cyclin-D related, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related, eight twenty one protein, myeloid translocation gene on 8q22, runt related transcription factor 1; translocated to, 1 (cyclin D related), zinc finger MYND domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1T1/ETO antigen affinity purified polyclonal antibody (Catalog # A01792-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1T1/ETO at approximately 67KD. The expected band size for RUNX1T1/ETO is at 67KD.
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