
Western blot analysis of various lysates using MERS-CoV Spike RBD Rabbit pAb (TA378465) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
S Protein Rabbit Polyclonal Antibody
TA378465
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierOriGene
- Product NameS Protein Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- HostRabbit
- IsotypeIgG
- Protein IDK9N5Q8
- Protein NameSpike glycoprotein
- Scientific DescriptionMERS-CoV Spike RBD Rabbit polyclonal Antibody
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203



![Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635866/GTX635866_44179_20210712_Neutralizing_Inhibition_w_23061202_325.webp)


