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ProductsBack/S100B / S100 Beta Antibody

S100B / S100 Beta Antibody

LS-C812338
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetS100B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    S100B / S100 Beta Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6285
  • Target name
    S100B
  • Target description
    S100 calcium binding protein B
  • Target synonyms
    NEF; protein S100-B; S100; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100-B; S100beta
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100B using anti-S100B antibody (M00979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100B antigen affinity purified monoclonal antibody (Catalog # M00979-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100B at approximately 11 kDa. The expected band size for S100B is at 11 kDa.
Product group Antibodies
References
Boster Bio
Anti-S100B/S100 Beta Rabbit Monoclonal AntibodyM00979-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetS100B
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Product group Antibodies
References
GeneTex
S100 beta antibodyGTX129573
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetS100B
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Product group Antibodies
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S100B AntibodyCAB0676
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S100 Calcium Binding Protein B (S100B) Monoclonal AntibodyCAU35023
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TargetS100B
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Product group Antibodies
LifeSpan BioSciences
S100B / S100 Beta Antibody (Preservative Free)LS-C149156
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA020643ESR1HU at dilution of 1:100
Product group Antibodies
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ReactivityHuman
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RayBiotech
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Immunohistochemistry analysis in human cerebral cortex and kidney tissues using AMAb91038 antibody. Corresponding S100B RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
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ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetS100B
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Everest Biotech
Goat anti-S100BEB12316
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TargetS100B
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