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SAMSN1 Antibody (Preservative Free)

LS-C815773
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSAMSN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SAMSN1 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64092
  • Target name
    SAMSN1
  • Target description
    SAM domain, SH3 domain and nuclear localization signals 1
  • Target synonyms
    HACS1; hematopoietic adapter-containing SH3 and sterile alpha-motif (SAM) domains 1; hematopoietic adapter-containing SH3 and sterile I+/--motif (SAM) domains 1; hematopoietic adaptor containing SH3 and SAM domains 1; NASH1; nuclear localization signals, SAM and SH3 domain containing 1; SAM and SH3 domain containing 2; SAM domain, SH3 domain and nuclear localisation signals, 1; SAM domain, SH3 domain and nuclear localization signals protein 1; SAM domain-containing protein SAMSN-1; SASH2; SH3D6B; SH3-SAM adaptor protein; SLy2; Src homology domain 3 (SH3)-containing adapter protein SH3 lymphocyte protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMSN1 antigen affinity purified polyclonal antibody (Catalog # A08977-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAMSN1 at approximately 50 kDa. The expected band size for SAMSN1 is at 42 kDa.
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