More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SAPK3 (MAPK12) Mouse Monoclonal Antibody [Clone ID: OTI10E1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK12 (Cat# RC204857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12 (Cat# TA500500). Positive lysates [LY401039] (100ug) and [LC401039] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK12 (Cat# RC204857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12 (Cat# TA500500). Positive lysates [LY401039] (100ug) and [LC401039] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK12 (Cat# RC204857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12 (Cat# TA500500). Positive lysates [LY401039] (100ug) and [LC401039] (20ug) can be purchased separately from OriGene.

SAPK3 (MAPK12) Mouse Monoclonal Antibody [Clone ID: OTI10E1]

CF500500
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK12
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    SAPK3 (MAPK12) Mouse Monoclonal Antibody [Clone ID: OTI10E1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI10E1
  • Gene ID6300
  • Target name
    MAPK12
  • Target description
    mitogen-activated protein kinase 12
  • Target synonyms
    ERK-6, ERK3, ERK6, MAPK 12, P38GAMMA, PRKM12, SAPK-3, SAPK3, mitogen-activated protein kinase 12, MAP kinase 12, MAP kinase p38 gamma, extracellular signal-regulated kinase 6, mitogen-activated protein kinase 3, mitogen-activated protein kinase p38 gamma, stress-activated protein kinase 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP53778
  • Protein Name
    Mitogen-activated protein kinase 12
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MAPK12 mouse monoclonal antibody, clone OTI10E1 (formerly 10E1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
MAPK12 AntibodyCAB13046
ReactivityHuman
TargetMAPK12
  • SizePrice
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Mouse skeletal muscle tissue All lanes: MAPK12 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41 kDa Observed band size: 42 kDa
Product group Antibodies
Cusabio
MAPK12 AntibodyCSB-PA013451LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAPK12
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MAPK12 Antibody144-60159
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAPK12
  • SizePrice
Anti-MAPK12 Antibody
Product group Antibodies
Antibodies.com
Anti-MAPK12 AntibodyA37349
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAPK12 / ERK6 / SAPK3 AntibodyLS-C748111
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAPK12
  • SizePrice
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-MAPK12 AntibodyHPA054562
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAPK12
  • SizePrice
Figure 1. Western blot analysis of P38 Gamma/MAPK12 using anti-P38 Gamma/MAPK12 antibody (A03942-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Gamma/MAPK12 antigen affinity purified polyclonal antibody (Catalog # A03942-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Gamma/MAPK12 at approximately 42 kDa. The expected band size for P38 Gamma/MAPK12 is at 42 kDa.
Product group Antibodies
Boster Bio
Anti-p38 gamma/MAPK12 Antibody Picoband(r)A03942-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAPK12
  • SizePrice
WB analysis of HEK293T cells transfected with p38 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX84148 p38 gamma antibody [10E1]. Loading : 5 ug per lane
Product group Antibodies
GeneTex
p38 gamma antibody [10E1]GTX84148
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAPK12
  • SizePrice
Product group Antibodies
Biomatik
MAPK12 Polyclonal AntibodyCAC14739
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetMAPK12
  • SizePrice