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ProductsBack/SCARB1 / SR-BI Antibody (aa478-509)

SCARB1 / SR-BI Antibody (aa478-509)

LS-C407715
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Mouse, Rat
TargetSCARB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCARB1 / SR-BI Antibody (aa478-509)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID949
  • Target name
    SCARB1
  • Target description
    scavenger receptor class B member 1
  • Target synonyms
    CD36L1, CLA-1, CLA1, HDLCQ6, HDLQTL6, SR-BI, SRB1, scavenger receptor class B member 1, CD36 and LIMPII analogous 1, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1, scavenger receptor class B type III
  • Host
    Rabbit
  • Reactivity
    Hamster, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Scavenging Receptor SR-BI/SCARB1 using anti-Scavenging Receptor SR-BI/SCARB1 antibody (A01093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 antigen affinity purified polyclonal antibody (Catalog # A01093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scavenging Receptor SR-BI/SCARB1 at approximately 85 kDa. The expected band size for Scavenging Receptor SR-BI/SCARB1 is at 85 kDa.
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