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Western blot analysis of extracts of various cell lines using SCYL2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SCYL2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SCYL2 Polyclonal Antibody at dilution of 1:3000.

SCYL2 Polyclonal Antibody

E-AB-64489
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SCYL2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCYL2 using anti-SCYL2 antibody (A08578-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL2 antigen affinity purified polyclonal antibody (Catalog # A08578-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL2 at approximately 104 kDa. The expected band size for SCYL2 is at 104 kDa.
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