Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20221216_WB_Z_22122018_588.webp "Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![SDHA antibody [HL1067] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA stained by SDHA antibody [HL1067] (GTX636098) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20211008_ICC_IF_w_23061202_852.webp "SDHA antibody [HL1067] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA stained by SDHA antibody [HL1067] (GTX636098) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20211008_WB_w_23061202_766.webp "Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20211008_WB_shRNA_watermark_w_23061202_461.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20211008_WB_M_R_w_23061202_249.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20230630_WB_Drosophila_23062718_911.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole Japanese medaka extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636098/GTX636098_44438_20250815_WB_medaka_25082121_399.webp "Whole Japanese medaka extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDHA antibody [HL1067]
GTX636098
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat, Zebra Fish
TargetSDHA
Overview
- SupplierGeneTex
- Product NameSDHA antibody [HL1067]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1067
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6389
- Target nameSDHA
- Target descriptionsuccinate dehydrogenase complex flavoprotein subunit A
- Target synonymsCMD1GG, FP, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF, succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, flavoprotein subunit of complex II, malate dehydrogenase [quinone] flavoprotein subunit, succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
- HostRabbit
- IsotypeIgG
- Protein IDP31040
- Protein NameSuccinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
- Scientific DescriptionThis gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
- ReactivityDrosophila, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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