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Search results: 19-c03

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Western blot analysis of Phospho-alpha Synuclein (Ser129) expression in (1) 293T cell lysate; (2) 293T cell lysate transfected with Polo-Like Kinase 2 and alpha Synuclein.
Product group Antibodies
Boster Bio
Anti-Phospho-alpha Synuclein (S129) SNCA Rabbit Monoclonal AntibodyP00215-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSNCA
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1) SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal AntibodyP01423-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate (P01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 monoclonal antibody (Catalog # P01423) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5
Product group Antibodies
Boster Bio
Anti-Phospho-Smad1/5/9 (S463/S465/S467) Rabbit Monoclonal AntibodyP01423
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Western blot analysis of Phospho-Synapsin I (S9) expression in (1) Human brain lysate; (2) Human brain lysate treated with AP (P03794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYN1 monoclonal antibody (Catalog # P03794) overnight at 4 °C; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYN1
Product group Antibodies
Boster Bio
Anti-Phospho-Synapsin I (S9) Rabbit Monoclonal AntibodyP03794
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSYN1
  • SizePrice
Western blot analysis of SIN3A expression in (1) K562 cell lysate; (2) RAW 264.7 cell lysate using anti-SIN3A antibody (M01203). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIN3A antigen affinity purified polyclonal antibody (Catalog # M01203) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SIN3A.
Product group Antibodies
Boster Bio
Anti-mSin3A Rabbit Monoclonal AntibodyM01203
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSIN3A
  • SizePrice
Western blot analysis of SERPINF1 expression in K562 cell lysate using anti-SERPINF1 antibody (M02034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINF1 antigen affinity purified polyclonal antibody (Catalog # M02034) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SERPINF1.
Product group Antibodies
Boster Bio
Anti-PEDF Monoclonal AntibodyM02034
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetSERPINF1
  • SizePrice
Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
Product group Antibodies
Boster Bio
Anti-Nesprin 1 Rabbit Monoclonal AntibodyM02192
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSYNE1
  • SizePrice
Western blot analysis of GLUT3 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate (M03259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A3 monoclonal antibody (Catalog # M03259) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A3
Product group Antibodies
Boster Bio
Anti-GLUT3 SLC2A3 Rabbit Monoclonal AntibodyM03259
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSLC2A3
  • SizePrice
Western blot analysis of gamma Synuclein expression in human fetal brain lysate.
Product group Antibodies
Boster Bio
Anti-gamma Synuclein Rabbit Monoclonal AntibodyM03523
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetSNCG
  • SizePrice
Western blot analysis of GCET1 expression in MCF7 cell lysate (M09606). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA9 monoclonal antibody (Catalog # M09606) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA9
Product group Antibodies
Boster Bio
Anti-GCET1 SERPINA9 Rabbit Monoclonal AntibodyM09606
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSERPINA9
  • SizePrice
Western blot analysis of SOX1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-SOX1 Rabbit Monoclonal AntibodyM08724
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSOX1
  • SizePrice
Western blot analysis of SCGN using anti-SCGN antibody (M10629) in human fetal brain lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCGN antigen affinity purified polyclonal antibody (Catalog # M10629) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-SCGN Monoclonal AntibodyM10629
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSCGN
  • SizePrice
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