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Western blot analysis of CAD using anti-CAD antibody (A00463-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.
Product group Antibodies
Boster Bio
Anti-CAD/CAD AntibodyA00463-1
Product group Antibodies
Biorbyt
CAD antibodyORB625357
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCAD
  • SizePrice
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells.
Product group Antibodies
Atlas Antibodies
Anti-CADHPA069341
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCAD
  • SizePrice
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-CADHPA057266
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCAD
  • SizePrice
GTX55350 IHC-P Image
Product group Antibodies
GeneTex
CAD (phospho Thr456) antibodyGTX55350
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CAD Antibody (N-Terminus)LS-B11936
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Hamster, Human, Mammals, Mouse, Rat
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
CAD Antibody Blocking PeptideLS-E754
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
CAD Antibody Blocking PeptideLS-E16807
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
CAD Antibody Blocking PeptideLS-E17342
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CAD Antibody (aa2018-2067)LS-C80826
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Zebra Fish
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CAD Antibody (aa238-287)LS-C81123
ApplicationsWestern Blot
ReactivityDrosophila
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CAD Antibody (phospho-Thr456)LS-C825545
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
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