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Search results: DHFR

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Western blot analysis of DHFR using anti-DHFR antibody (M00813) in (1) HeLa cell lysate; (2) Mouse spleen lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # M00813) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-DHFR Monoclonal AntibodyM00813
IHC analysis of DHFR using anti-DHFR antibody (PB9175).
Product group Antibodies
Boster Bio
Anti-DHFR Picoband AntibodyPB9175
Product group Antibodies
Reddot Biotech
DHFR Polyclonal AntibodyRD252839A
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
More sizes
Product group Antibodies
Reddot Biotech
DHFR Polyclonal AntibodyRD82229A
ApplicationsImmunoFluorescence
ReactivityHuman, Mouse
TargetDHFR
  • SizePrice
Product group Antibodies
Reddot Biotech
DHFR Polyclonal AntibodyRD218927A
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
More sizes
Product group Chemicals
MedChem Express
DHFR-IN-10HY-156412
Product group Chemicals
MedChem Express
DHFR-IN-11HY-156414
GTX84617 ICC/IF Image
Product group Antibodies
GeneTex
DHFR antibody [6G7] - Overexpression ValidatedGTX84617
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Monkey
  • SizePrice
GTX84618 IHC-P Image
Product group Antibodies
GeneTex
DHFR antibody [3A11] - Overexpression ValidatedGTX84618
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Monkey, Mouse, Rat
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Mouse DHFR Protein (Recombinant His)LS-G1134
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Human DHFR Protein (Recombinant His)LS-G592
  • SizePrice
GTX110710-pro Image
Product group Molecular Biology
GeneTex
Human DHFR protein, His tagGTX110710-PRO100
  • SizePrice
56789

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