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Search results: GPHN

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Lane 1: Lung lysates probed with Gephyrin Polyclonal Antibody, Unconjugated (bs-6644R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetGPHN
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Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGPHN
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Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGPHN
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Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
ReactivityChicken, Human, Mouse, Rat
TargetGPHN
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Product group Antibodies
Bethyl Laboratories
ApplicationsWestern Blot
ReactivityChicken, Human, Mouse, Rat
TargetGPHN
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Product group Antibodies
Absolute Antibody
Anti-Gephyrin [GC1]AB02267-10.0-BT
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGPHN
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Figure 1. Western blot analysis of Gephyrin using anti-Gephyrin antibody (M04560-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gephyrin antigen affinity purified monoclonal antibody (Catalog # M04560-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gephyrin at approximately 93 kDa. The expected band size for Gephyrin is at 80 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGPHN
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetGPHN
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetGPHN
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetGPHN
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetGPHN
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetGPHN
  • SizePrice