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Search results: IL17B

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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-DYLIGHT594
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-FITC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-HRP
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C310742
ApplicationsWestern Blot
ReactivityHuman
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C662281
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C662806
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C666382
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C778544
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C104685
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL17B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL17B AntibodyLS-C807511
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetIL17B
  • SizePrice
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