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Search results: IL17B

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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
  • SizePrice
Product group Proteins / Signaling Molecules
Antibodies.com
Recombinant Human IL-17B Protein (N-terminal His Tag)A61491
ApplicationsOther Application
  • SizePrice
WB analysis of GTX00134-pro Human IL17B protein (active).
Product group Proteins / Signaling Molecules
GeneTex
Human IL17B protein, His tag (active)GTX00134-PRO
ApplicationsFunctional Assay
Protein IDQ9UHF5
  • SizePrice
Product group Antibodies
7BioScience
Anti-Human IL-17B7B-AHIL17B
  • SizePrice
Product group Antibodies
Sino Biological
Anti-IL17B Antibody, Rabbit Polyclonal201270-T40
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • SizePrice
Product group Antibodies
Biomatik
Interleukin 17B (IL17B) Polyclonal AntibodyCAU32942
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse
TargetIl17b
  • SizePrice
Product group Molecular Biology
Abbexa
Mouse IL17B shRNA PlasmidABX974610
  • SizePrice
Product group Molecular Biology
Abbexa
Human IL17B shRNA PlasmidABX959017
  • SizePrice
Product group Assays
Biomatik
Human Interleukin 17B (Il17B) CLIA KitEKU09697
Assay Sample TypeSerum, plasma and other biological fluids
ReactivityHuman
  • SizePrice
Product group Assays
Biomatik
Mouse Interleukin 17B (Il17B) CLIA KitEKU09698
Assay Sample TypeSerum, plasma and other biological fluids
ReactivityMouse
  • SizePrice
Product group Assays
Biomatik
Human Interleukin 17B (Il17B) ELISA KitEKU05241
Assay Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
ReactivityHuman
  • SizePrice
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