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Search results: IL17B

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Product group Lysates / Extracts
Sino Biological
Mouse IL17B Baculovirus-Insect cells Overexpression Lysate51304-M02BL
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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2
ApplicationsWestern Blot, ELISA
TargetIL17B
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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3
ApplicationsWestern Blot, ELISA
TargetRNU4-7P
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Product group Antibodies
RayBiotech
Anti-IL17B (Center) Antibody102-20997
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetIL17B
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Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen IL17BAPREST74079
Protein IDQ9UHF5
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Product group Proteins / Signaling Molecules
Biomatik
Recombinant Rat Interleukin 17B (IL17B)RPU42250
ApplicationsWestern Blot, Other Application
Protein IDG3V8K9
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Product group Proteins / Signaling Molecules
Biomatik
Recombinant Human Interleukin 17B (IL17B)RPU42251
ApplicationsWestern Blot, Other Application
Protein IDQ9UHF5
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Product group Body Products
7BioScience
Human IL-17B7B-HIL17B
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Product group Assays
Biomatik
Human Interleukin17B (Il17B) ELISA KitEKC42340
Assay Sample Typeserum, plasma, cell culture supernates
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Product group Antibodies
Biomatik
Interleukin 17B (IL17B) Polyclonal AntibodyCAU25468
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIl17b
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Product group Antibodies
Biomatik
Interleukin 17B (IL17B) Polyclonal AntibodyCAU25469
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIL17B
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Product group Proteins / Signaling Molecules
Biomatik
Human Interleukin 17B (IL17B), Active ProteinRPU54938
ApplicationsOther Application
Protein IDQ9UHF5
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