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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-3-PE
ApplicationsWestern Blot, ELISA
TargetRNU4-7P
  • SizePrice
Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen IL17BAPREST74079
Protein IDQ9UHF5
  • SizePrice
Product group Antibodies
Abbexa
Interleukin 17B (IL17B) AntibodyABX038115
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Active Interleukin 17B (IL17B)APB700HU01
Protein IDQ9UHF5
  • SizePrice
Product group Antibodies
Abbexa
Interleukin 17B (IL17B) AntibodyABX330118
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Interleukin 17B (IL17B)RPB700HU01
ApplicationsWestern Blot, Other Application
Protein IDQ9UHF5
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Interleukin 17B (IL17B)RPB700RA01
ApplicationsWestern Blot, Other Application
Protein IDG3V8K9
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2-BIOTIN
ApplicationsWestern Blot, ELISA
TargetIL17B
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetIL17B
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2-CY3
ApplicationsWestern Blot, ELISA
TargetIL17B
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2-DYLIGHT488
ApplicationsWestern Blot, ELISA
TargetIL17B
  • SizePrice
Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B Antibody Picoband(r)A10846-2-DYLIGHT550
ApplicationsWestern Blot, ELISA
TargetIL17B
  • SizePrice
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