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Search results: LOX CRISPR

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Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-LOX Antibody Picoband(r)PB9718-FITC
ApplicationsWestern Blot, ELISA
ReactivityHamster, Human, Mouse, Rat
TargetLOX
  • SizePrice
Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-LOX Antibody Picoband(r)PB9718-HRP
ApplicationsWestern Blot, ELISA
ReactivityHamster, Human, Mouse, Rat
TargetLOX
  • SizePrice
Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-LOX Antibody Picoband(r)PB9718-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityHamster, Human, Mouse, Rat
TargetLOX
  • SizePrice
Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-LOX Antibody Picoband(r)PB9718-PE
ApplicationsWestern Blot, ELISA
ReactivityHamster, Human, Mouse, Rat
TargetLOX
  • SizePrice
Figure 1. Western blot analysis of LOX using anti-LOX antibody (PB9718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa.
Product group Antibodies
References
Boster Bio
Anti-LOX Antibody Picoband(r)PB9718
ApplicationsWestern Blot, ELISA
ReactivityHamster, Human, Mouse, Rat
TargetLOX
  • SizePrice
Rat LOX 1 ELISA Kit
Product group Assays
Antibodies.com
Rat LOX 1 ELISA KitA2084
Assay Sample TypeSerum, plasma and other biological fluids.
ReactivityRat
  • SizePrice
Mouse LOX 1 ELISA Kit
Product group Assays
Antibodies.com
Mouse LOX 1 ELISA KitA2085
Assay Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
ReactivityMouse
  • SizePrice
Rabbit LOX 1 ELISA Kit
Product group Assays
Antibodies.com
Rabbit LOX 1 ELISA KitA2086
Assay Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
ReactivityRabbit
  • SizePrice
Mouse LOX 1 ELISA Kit
Product group Assays
Antibodies.com
Mouse LOX 1 ELISA KitA314108
ReactivityMouse
  • SizePrice
Human LOX ELISA Kit
Product group Assays
Antibodies.com
Human LOX ELISA KitA314293
ReactivityHuman
  • SizePrice
Human LOX ELISA Kit
Product group Assays
Antibodies.com
Human LOX ELISA KitA5213
Assay Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
ReactivityHuman
  • SizePrice
Mouse LOX ELISA Kit
Product group Assays
Antibodies.com
Mouse LOX ELISA KitA5453
Assay Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
ReactivityMouse
  • SizePrice
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