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Search results: LOX CRISPR

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Lox Mouse Gene Knockout Kit (CRISPR)
Product group Genome Editing and Engineering
OriGene
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Product group Assays
Assay Genie
Assay Sample TypeSerum, plasma and other biological fluids
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Product group Assays
Assay Genie
Assay Sample TypeSerum, plasma and other biological fluids
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Product group Assays
Assay Genie
Assay Sample TypeSerum, plasma and other biological fluids
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LOX Human Gene Knockout Kit (CRISPR)
Product group Genome Editing and Engineering
OriGene
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Lox Mouse Gene Knockout Kit (CRISPR)
Product group Genome Editing and Engineering
OriGene
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Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetOLR1
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Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human prostatic carcinoma tissue (PCT) lysates, Lane 2: human cervical carcinoma tissue (CCAT) lysates, Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1833). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human prostatic carcinoma tissue (PCT) lysates, Lane 2: human cervical carcinoma tissue (CCAT) lysates, Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1833) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetOLR1
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice