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Search results: MAP2K1 MEK1

337 to 348 of 3.106 products
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Recombinant Mouse MEK1/MAP2K1/MKK1 Protein (Active)
Product group Proteins / Signaling Molecules
Elabscience
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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Human, Mouse, Primate, Rat, Xenopus, Zebra Fish
TargetMAP2K1
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Assays
LifeSpan BioSciences
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Figure 1. Immunohistochemistry validation of MAP2K1 using Anti-MEK1/2 (phospho-S222/226) MAP2K1 Antibody (A00292S222). Immunohistochemistry (IHC) analyzes of p-MEK1/2 (S222/226) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. For more protocol information of IHC
Product group Antibodies
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
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Western blot analysis of MEK5 expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice

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