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Search results: MAP2K1 MEK1

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Western blot analysis of various lysates using Phospho-MEK1-T286 Rabbit pAb (TA378246) at 1:2000 dilution or MEK1 antibody (A12687). HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by ATP(5 mM) at 30℃ for 1 hour. PC-12 cells were treated by Nocodazole (1 μg/mL) at 37℃ for 16 hours or treated by ATP(5 mM) at 30℃ for 1 hour.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 30s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378246
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot (WB) analysis of MEK1 (Phospho-S298) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane4:HeLa whole cell lysate(40ug)
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA392531
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of various lysates using [KO Validated] MEK1 Rabbit pAb (TA378242) at 1:3000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit .<br/>Exposure time: 90s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378242
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 in (1) 293T lysates; (2) A549 lysates using MEK1/2 antibody.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA384707
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of lysates from HeLa cells
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378247
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of lysates from PC-12 cells
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378248
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetMAP2K1
  • SizePrice
Western blot (WB) analysis of MEK1 (Ser298) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug) Lane6:CT26 whole cell lysate(40ug)
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA392532
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot (WB) analysis of p-MEK1 (S298) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:LOVO whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug) Lane6:HCT116 whole cell lysate(40ug)
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA392651
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (TA378244) at 1:1000 dilution or MEK1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30℃ for 1 hour.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 1s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378244
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of lysates from C6 cells, using Phospho-MEK1-pS221 Rabbit pAb (TA378245) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 60s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378245
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western Blot: MEK1 Antibody [TA336999] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lan
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA336999
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Drosophila, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA349182
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
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