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Search results: MAP2K1 MEK1

505 to 516 of 3.127 products
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Product group Antibodies
Biorbyt
MEK1 (MAP2K1) Antibody (N-term)ORB1788227
ApplicationsWestern Blot
TargetMAP2K1
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Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK1/2 MAP2K1 Rabbit Monoclonal AntibodyM00292-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K1
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Product group Lysates / Extracts
LifeSpan BioSciences
MAP2K1 / MKK1 / MEK1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length)LS-G99857
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Mouse MAP2K1 / MKK1 / MEK1 Protein (Recombinant His/GST,N-terminus) (Met1-Ile393)LS-G139937
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Product group Antibodies
Assay Genie
MEK1/MEK2 AntibodyCAB18117
TargetICOS
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Product group Antibodies
Biosynth
MAP2K1 antibody (Thr386)70R-32676
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Product group Antibodies
Biosynth
MAP2K1 antibody (Thr292)70R-15010
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Product group Antibodies
Biosynth
MAP2K1 antibody (Thr386)70R-15011
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Product group Antibodies
Assay Genie
MEK1/MEK2 Monoclonal AntibodyCAB4868
TargetMAP2K1
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Recombinant Mouse MEK1/MAP2K1/MKK1 Protein (His & GST Tag)
Product group Proteins / Signaling Molecules
Elabscience
Recombinant Mouse MEK1/MAP2K1/MKK1 Protein (His & GST Tag)PKSM040747
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Product group Proteins / Signaling Molecules
Biorbyt
MEK1 (MAP2K1) (S218) Antibody Blocking peptideORB1445637
Protein IDQ02750
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Product group Molecular Biology
SignalChem
MEK1, UnactiveM02-14BG
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