Bio-Connect

Search results: MAP2K1 MEK1

505 to 516 of 3.082 products
Sort by
Figure 1. Western blot analysis of MEK1-2 using anti-MEK1-2 antibody (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1-2 antigen affinity purified monoclonal antibody (Catalog # M00292-1) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1-2 at approximately 43 kDa. The expected band size for MEK1-2 is at 43 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
  • SizePrice
Product group Antibodies
Assay Genie
TargetICOS
  • SizePrice
Product group Antibodies
Assay Genie
TargetMAP2K1
  • SizePrice
Recombinant Mouse MEK1/MAP2K1/MKK1 Protein (His & GST Tag)
Product group Proteins / Signaling Molecules
Elabscience
  • SizePrice
Product group Proteins / Signaling Molecules
Biorbyt
Protein IDQ02750
  • SizePrice
Product group Molecular Biology
SignalChem
  • SizePrice
Product group Molecular Biology
SignalChem
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Western blot (WB) analyzes of MEK1/2 antibody (Cat.-No.: AP20701PU-N) in extracts from hela and raw264.7 cells.
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
Western blot analyzes of MEK1/2 antibody (Cat.-No.: AP20702PU-N) in extracts from HT-29 cells.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice