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Search results: MAP2K1 MEK1

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Product group Antibodies
Biosynth
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IHC image of CSB-PA019284LA01HU diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
RAF1 AntibodyCSB-PA019284LA01HU
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRAF1
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetRAF1
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Product group Antibodies
Cusabio
ReactivityHuman
TargetRAF1
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetRAF1
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Western Blot Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF) All lanes Phospho-RAF1 antibody at 1.18microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 KDa Observed band size: 73 KDa
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRAF1
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Immunofluorescence staining of Hela with CSB-RA019284A43phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetRAF1
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