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Search results: MAP2K1 MEK1

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Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate (P00292-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-3) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-Phospho-MEK1 (S298) MAP2K1 Rabbit Monoclonal AntibodyP00292-3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Ser298) antibody [GT1305] (GTX03217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
MEK1 (phospho Ser298) antibody [GT1305] - Orthogonal ValidatedGTX03217
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Thr292) antibody [GT1306] (GTX03218) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibody [GT1306] - Orthogonal ValidatedGTX03218
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500
Product group Antibodies
GeneTex
MEK1 antibody [1F5] - KO/KD, Overexpression ValidatedGTX84167
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
MyBioSource
MAP2K1 (MAP Kinase Kinase 1, MMAP2K1, MAPKK1, MEK1, MKK1, PRKMK1) (MaxLight 405)MBS6425346
  • SizePrice
Product group Antibodies
MyBioSource
MAP2K1 (MAP Kinase Kinase 1, MMAP2K1, MAPKK1, MEK1, MKK1, PRKMK1) (MaxLight 490)MBS6425347
  • SizePrice
Product group Antibodies
MyBioSource
MAP2K1 (MAP Kinase Kinase 1, MMAP2K1, MAPKK1, MEK1, MKK1, PRKMK1) (MaxLight 550)MBS6425348
  • SizePrice
Product group Antibodies
MyBioSource
MAP2K1 (MAP Kinase Kinase 1, MMAP2K1, MAPKK1, MEK1, MKK1, PRKMK1) (MaxLight 650)MBS6425349
  • SizePrice
Product group Antibodies
MyBioSource
MAP2K1 (MAP Kinase Kinase 1, MMAP2K1, MAPKK1, MEK1, MKK1, PRKMK1) (MaxLight 750)MBS6425350
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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K1 AntibodyHPA026430
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
MAP2K1 antibodyORB48687
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biorbyt
MAP2K1 antibodyORB519171
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
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