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Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-CARRIER-FREE
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-CY3
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-DYLIGHT488
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-DYLIGHT550
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-DYLIGHT594
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-FITC
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-HRP
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-IFLUOR647
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499-PE
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2/MAP3K2 using anti-MEKK2/MAP3K2 antibody (A02354-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2/MAP3K2 antigen affinity purified polyclonal antibody (Catalog # A02354-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2/MAP3K2 at approximately 78KD. The expected band size for MEKK2/MAP3K2 is at 78KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)A02354-1-BIOTIN
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2/MAP3K2 using anti-MEKK2/MAP3K2 antibody (A02354-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2/MAP3K2 antigen affinity purified polyclonal antibody (Catalog # A02354-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2/MAP3K2 at approximately 78KD. The expected band size for MEKK2/MAP3K2 is at 78KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)A02354-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetMAP3K2
  • SizePrice
Figure 1. Western blot analysis of MEKK2/MAP3K2 using anti-MEKK2/MAP3K2 antibody (A02354-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2/MAP3K2 antigen affinity purified polyclonal antibody (Catalog # A02354-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2/MAP3K2 at approximately 78KD. The expected band size for MEKK2/MAP3K2 is at 78KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)A02354-1-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetMAP3K2
  • SizePrice
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