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Search results: MEKK2

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Product group Molecular Biology
SignalChem
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WB analysis of various samples using GTX11792 MEKK2 antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat testis tissue lysate Lane 3 : rat lung tissue lysate
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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Product group Antibodies
MedChem Express
TargetMAP3K2
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ELISA analysis of antigen using GTX60504 MEKK2 antibody [4B4]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetMAP3K2
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Product group Chemicals
MedChem Express
Molecular WeightApproximately 96.5 kDa
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MEKK2, GST-tag Recombinant
Product group Molecular Biology
BPS Bioscience
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Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
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Product group Antibodies
Assay Genie
ReactivityMouse
TargetMAP3K2
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Figure 2. Immunocytochemistry staining of MAP3K2 using Anti-MEKK2 MAP3K2 Antibody (A02354). ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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Flow cytometric analysis of HepG2 cells with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
  • SizePrice