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Search results: MSL2

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Western Blot Positive WB detected in: U251 whole cell lysate All lanes: MSL2 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63, 55 kDa Observed band size: 63 kDa
Product group Antibodies
Cusabio
MSL2 AntibodyCSB-PA875722LA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMSL2
  • SizePrice
Western blot analysis in human cell line MOLT-4.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetMSL2
  • SizePrice
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMSL2
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA
TargetMSL2
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsELISA
ReactivityHuman
TargetMSL2
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetMSL2
  • SizePrice
Product group Antibodies
Cusabio
ReactivityHuman
TargetMSL2
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetMSL2
  • SizePrice
Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetMSL2
  • SizePrice
Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetMSL2
  • SizePrice
Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetMSL2
  • SizePrice