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Search results: PDCD4

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Lane 1: Hela cell lysates; Lane 2: PDCD4 knockout (KO) HeLa cell lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPDCD4
  • SizePrice
Formalin-fixed and paraffin embedded human brain glioblastoma labeled Anti-PDCD4 Polyclonal Antibody, Unconjugated (bs-1608R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPDCD4
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3microg Human PDCD4 protein (GTX68403-pro) by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Proteins / Signaling Molecules
GeneTex
Protein IDQ53EL6
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Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPDCD4
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Product group Proteins / Signaling Molecules
Everest Biotech
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Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice
3microg Human PDCD4 protein (GTX68404-pro) by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Proteins / Signaling Molecules
GeneTex
Protein IDQ53EL6
  • SizePrice
Product group Chemicals
Biorbyt
PDCD4-IN-1ORB1745158
Estimated Purity99.21%
Molecular Weight326.58
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Product group Proteins / Signaling Molecules
Biorbyt
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Product group Antibodies
Assay Genie
ReactivityHuman
TargetPDCD4
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Product group Assays
Biorbyt
ReactivityHuman
  • SizePrice
Anti-PDCD4 Antibody [ARC1398]
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice