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Search results: PDCD4

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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Product group Proteins / Signaling Molecules
Boster Bio
ApplicationsOther Application
Protein IDQ53EL6
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetPDCD4
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Western blot analysis of extracts from 293 cells treated with Serum using Phospho-PDCD4 (Ser457) antibody.The lane on the right is treated with the antigen-specific peptide.
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Western blot analysis of extracts from HepG2 tissue using Phospho-PDCD4 (Ser67) antibody.The lane on the right is treated with the antigen-specific peptide.
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
IF(ICC) staining with PDCD4 (3G1) Monoclonal Antibody (bsm-54041R) at 1:50 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetPDCD4
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WB analysis of Jurkat cells using GTX52831 PDCD4 antibody at 0.2-1microg/ml.
Product group Antibodies
References
GeneTex
ApplicationsWestern Blot
ReactivityHuman
TargetPDCD4
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetPDCD4
  • SizePrice
Western Blot analysis of 293 cells using Phospho-Pdcd-4 (S457) Polyclonal Antibody
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPDCD4
  • SizePrice
Product group Assays
Assay Genie
Assay Sample TypeSerum, Plasma
ReactivityHuman
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD4
  • SizePrice