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Western blot analysis of extracts from 293 cells treated with Serum using Phospho-PDCD4 (Ser457) antibody.The lane on the right is treated with the antigen-specific peptide.
Product group Antibodies
Cusabio
Phospho-PDCD4 (Ser457) AntibodyCSB-PA785011
ApplicationsWestern Blot, ELISA
TargetPDCD4
  • SizePrice
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
Anti-PDCD4 Rabbit Monoclonal AntibodyM01105
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-CY3
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-FITC
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-HRP
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60 kDa. The expected band size for PDCD4 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-PDCD4 Antibody Picoband(r)A01105-PE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetPDCD4
  • SizePrice
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