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Search results: STAT3

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Western blot analysis of STAT3 expression in Raji cell lysate (M00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # M00007-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Raji cell lysate.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Plant, Rat
TargetSTAT3
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Xenopus, Zebra Fish
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetSTAT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetSTAT3
  • SizePrice
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