
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX133464) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
STAT3 (phospho Tyr705) antibody
GTX133464
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSTAT3
Overview
- SupplierGeneTex
- Product NameSTAT3 (phospho Tyr705) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID6774
- Target nameSTAT3
- Target descriptionsignal transducer and activator of transcription 3
- Target synonymsADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3, DNA-binding protein APRF, acute-phase response factor
- HostRabbit
- IsotypeIgG
- Protein IDP40763
- Protein NameSignal transducer and activator of transcription 3
- Scientific DescriptionThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Fan JJ, Hsu WH, Lee KH, et al. Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling. Antioxidants (Basel). 2019,8(11). doi: 10.3390/antiox8110557Read this paper
- Shen M, Xu Z, Xu W, et al. Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway. J Exp Clin Cancer Res. 2019,38(1):149. doi: 10.1186/s13046-019-1161-8Read this paper
- Li X, Ding R, Han Z, et al. Targeting of cell cycle and let-7a/STAT3 pathway by niclosamide inhibits proliferation, migration and invasion in oral squamous cell carcinoma cells. Biomed Pharmacother. 2017,96:434-442. doi: 10.1016/j.biopha.2017.09.149Read this paper
- Liu H, Chang JK, Hou JQ, et al. Inhibition of miR-221 influences bladder cancer cell proliferation and apoptosis. Eur Rev Med Pharmacol Sci. 2017,21(14):3193-3199.Read this paper




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![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104616/GTX104616_43873_20210226_WB_R_w_23060120_540.webp)
